(2006 - 2010)
Rapid determination of particle velocity from space-time images using the Radon transform.
P. J. Drew, P. Blinder, G. Cauwenberghs, A. Y. Shih and D. Kleinfeld,
under review.
Phase-to-rate transformations in individual neurons encode
touch within a vibrissa scanning sensorimotor system.
J. Curtis and D. Kleinfeld, under rereview.
A proposal for a coordinated effort for the determination of brainwide neuroanatomical
connectivity in model organisms at a mesoscopic scale. J. W. Bohland, C. Wu, H. Barbas,
H. Bokil, H. T. Cline, P. J. Freed, R. J. Greenspan, S. N. Haber, M. Hawrylycz, D. G. Herrera,
C. C. Hilgetag, Z. J. Huang, A. Jones, E. G. Jones, H. J. Karten, D. Kleinfeld, R. Kötter,
H. A. Lester, J. M. Lin, S. Mikula, J. Panksepp, J. Safdieh, C. B. Saper, N. D. Schiff,
B. W. Stillman, K. Svoboda, L. W. Swanson, A. W. Toga, J. D. Watson and P. P. Mitra,
under review.
Severe blood brain barrier disruption and surrounding tissue integrity.
B. Chen, B. Friedman, Q. Cheng, E. Schim, D. Kleinfeld and P. D. Lyden,
under rereview.
Acute vascular disruption and Aquaporin 4 loss
after stroke. B. Friedman, C. Schachtrup, P. S. Tsai,
A. Y. Shih, K. Akassaglou, D. Kleinfeld and P. D. Lyden, Stroke (2009) in press.
PDF (11.0 Mb)
Preliminary aspects of this work were the subject of
a news release at the 2007 Society for Neuroscience Annual Meeting.
Active dilation of penetrating arterioles restores red blood cell
flux to penumbral neocortex after focal stroke. A. Y. Shih, B.
Friedman, P. J. Drew, P. S. Tsai, P. D. Lyden and D. Kleinfeld, Journal of Cerebral
Blood Flow and Metabolism (2009) in press.
PDF (3.1 Mb)
Plasma-mediated ablation: An optical tool for submicrometer surgery on
neuronal and vascular systems. P. S. Tsai, P. Blinder, B. J. Migliori,
J. Neev, Y. Jin, J. A. Squier and D. Kleinfeld, Current Opinion in Biotechnology (2009) in press.
PDF (1.0 Mb)
Stimulus-induced changes in blood flow and 2-deoxyglucose uptake dissociate in
ipsilateral somatosensory cortex. A. Devor, E. M. C.
Hillman, P. Tian, C. Waeber, I. C. Teng, L. Ruvinskaya, M. Shalinsky,
H. Zhu, R. H. Haslinger, S. N. Narayanan, I. Ulbert, A. K. Dunn,
E. H. Lo, B. R. Rosen, A. M. Dale, D. Kleinfeld and D. A. Boas, Journal of Neuroscience (2008) 28:14347-14357.
PDF (0.7 Mb)
Selected for This Week in the Journal, December 31, 2008.
PDF (0.1 Mb)
Advancing multifocal nonlinear microscopy: Development and application of a novel
multibeam Yb:KGd(WO4)2 oscillator. K. E. Sheetz, E. E. Hoover,
R. Carriles, D. Kleinfeld and J. A Squier,
Optics Express (2008) 16:17574-15584.
PDF (0.3 Mb)
Selected by the editor-in-chief, Gregory Faris, for publication in the
Virtual Journal for Biomedical Optics (http://vjbo.osa.org/virtual_issue.cfm).
Finding coherence in spontaneous oscillations.
P. J. Drew, J. H. Duyn, E. Golanov and D. Kleinfeld,
Nature Neuroscience (2008) 11:991-993.
PDF (0.7 Mb)
Texture coding in the rat whisker system:
Slip-stick versus differential resonance. J. Wolfe, D. N. Hill,
S. Pahlavan, P. J. Drew, D. Kleinfeld and D. E. Feldman, Public
Library of Science: Biology (2008) 6:1661-1677.
PDF (4.7 Mb)
Associated Primer "Whisker-Mediated Texture Discrimination" by M. E. Diamond,
M. von Heimendahl and E. Arabzadeh, Public Library of Science: Biology (2008) 6:1627-1630.
PDF (2.9 Mb)
“Where” and “What” in the whisker sensorimotor system.
M. E. Diamond, M. von Heimendahl, P. M. Knutsen, D. Kleinfeld
and E. Ahissar, Nature Reviews of Neuroscience (2008) 9:601-612.
PDF (1.1 Mb)
In vivo measurements of blood flow and glial cell function with
two-photon laser scanning microscopy. F. Helmchen and D. Kleinfeld,
Methods in Enzymology (2008) 444:231-254.
PDF (1.5 Mb)
Biomechanics of the vibrissa motor plant in rat: Rhythmic
whisking consists of triphasic neuromuscular activity.
D. N. Hill, R. Bermejo, H. P. Zeigler and D. Kleinfeld, Journal
of Neuroscience (2008) 28:3438-3455.
PDF (2.7 Mb); Simulation video MPG
(8.5 Mb)
Wilder Penfield in the age of YouTube: Visualizing the
sequential activation of sensorimotor areas across neocortex.
D. Kleinfeld and J. Waters, Neuron (2007) 56:760-762.
PDF (0.2 Mb)
Spherical aberration correction in nonlinear microscopy
and optical ablation using a transparent deformable membrane.
P. S. Tsai, B. Migliori, K. Campbell, T. Kim, Z. Kam,
A. Groisman and D. Kleinfeld, Applied Physics Letters (2007) 91:191102.
PDF (0.7 Mb);
Supplementary methods PDF
(0.4 Mb); News release, titled "An adjustable corrective
lens for two-photon microscopys " by H. Hogan, featured in
Biophotonics International. PDF
(0.5 Mb)
Suppressed neuronal activity and concurrent arteriolar
vasoconstriction may explain negative blood oxygenation
level-dependent signal[s]. A. Devor, P. Tian, N. Nishimura,
I. C. Teng, E. M. C. Hillman, S. N. Narayanan, I. Ulbert, D. A.
Boas, D. Kleinfeld and A. M. Dale, Journal of Neuroscience (2007)
27:4452-4459. PDF
(0.4 Mb)
Is there a common origin to surround-inhibition as seen
through electrical activity versus hemodynamic changes? Focus
on “Duration-dependent response in SI to vibrotactile stimulation”.
A. Devor, A. Trevelyan and D. Kleinfeld, Journal of
Neurophysiology (2007) 97:1880-1882. PDF
(0.1 Mb)
Large two-photon absorptivity of hemoglobin in the infrared
range of 780 - 880 nm. G. O. Clay, C. B. Schaffer and
D. Kleinfeld, Journal of Chemical Physics (2007) 126:025102. PDF
(0.3 Mb)
Active spatial perception in the vibrissa scanning sensorimotor
system. S. B. Mehta, D. Whitmer, R. Figueroa, B. A. Williams
and D. Kleinfeld, Public Library of Science: Biology (2007) 5:309-322.
PDF
(2.5 Mb);
supplementary methods PDF
(4.3 Mb)
Penetrating arterioles are a bottleneck in the perfusion
of neocortex. N. Nishimura, C. S. Schaffer, B. Friedman,
P. D. Lyden and D. Kleinfeld. Proceedings of the National Academy
of Sciences USA (2007) 104:365-370. PDF
(4.1 Mb);
supplementary methods PDF
(1.2 Mb)
News release, titled "Bottleneck In Blood Supply Makes Brain
Vulnerable To Strokes ", featured in Science Daily PDF
(0.1 Mb),
Medical News Today, Newswise, HULIQ.com, PHYSORG.com, SpiritIndia.com,
and MedIndia.com.
Active sensation: Insights from the rodent vibrissa sensorimotor
system. D. Kleinfeld, E. Ahissar and M. E. Diamond. Current
Opinions in Neurobiology (2006) 16:435-444. PDF
(0.2 Mb)
Laser photoablation: A new biomedical tool.
N. Nishimura, C. B. Schaffer, B. Friedman, P. S. Tsai, P. D. Lyden
and D. Kleinfeld. SPIE Newsroom (2006) http://spie.org/x8614.xml?highlight=x2416
PDF
(0.2 Mb)
Exploratory whisking by rat is not phase-locked to the
hippocampal theta rhythm. R. W. Berg, D. Whitmer and
D. Kleinfeld. Journal of Neuroscience (2006) 26:6518–6522.
PDF (0.5 Mb)
Seeing what the mouse sees with its vibrissae: A matter
of behavioral state. J. C. Curtis and D. Kleinfeld. Neuron
(2006) 50:524-526. PDF
(0.2 Mb)
MPScope: A versatile software suite for multiphoton microscopy.
Q.-T. Nguyen, P. S. Tsai and D. Kleinfeld, Journal of Neuroscience
Methods (2006) 156:351-359 PDF
(0.6 Mb).
Featured in "Research from University of California, Physics
Department yields new data on neuroscience'', Science Letter via
Technology Marketing Corporation (2007) PDF
(0.3 Mb).
Targeted insult to individual subsurface cortical blood
vessels using ultrashort laser pulses: Three models of stroke.
N. Nishimura, C. B. Schaffer, B. Friedman, P. S. Tsai, P. D. Lyden
and D. Kleinfeld, Nature Methods (2006) 3:99-108 PDF
(2.5 Mb);
supplemetary methods PDF
(1.3 Mb);
supplementary notes PDF
(0.1 Mb);
cover PDF
(0.3 Mb).
News and Views titled "Different strokes for rodent
folks"by R Flaumenhaft and E. H. Lo, Nature Methods (2006)
3:79-80 PDF
(0.6 Mb).
News release, titled "Stroke modeled with increased precision",
featured in Science Daily PDF
(0.5 Mb),
United Press International, Dementia.com, Psychiatry24x7.com,
Hum-Molgen News Alert, WebIndia, Post Chronicle, Biocompare Life
Science News, and Monsters & Critics News. Additionally featured
in "New bane for the rat brain" by K. Heyman in ScienceNow
(27 January 2006) PDF
(0.6 Mb),"Laser
lesions simulate microvessel stroke" by A. Burton in The
Lancet 5:206 PDF
(0.1 Mb),
and "Neurological disorders - Making steps in stroke therapy"
by S. Barton in Nature Reviews of Drug Discovery 5:187 and Nature
Reviews of Neuroscience 7:173 PDF
(0.1 Mb).
Preliminary results featured in "Femtosecond laser pulses
in biology: From microscopy to ablation and micromanipulation"
by M. Arrigoni, Biophotonics International (June 2004) 48-51 PDF
(0.7 Mb);
and in "Micro-scalpel offers unprecedented precision"
by K. Schmidt, New Scientist (25 March 2005) 40-45 PDF
(1.1 Mb).
Spectral mixing in nervous systems: Experimental evidence
and biologically plausible circuits. D. Kleinfeld and
S. B. Mehta. Progress of Theoretical Physics Supplement (2006)
161:86-98 PDF
(1.5 Mb).
Two-photon imaging of cortical surface microvessels reveals
a robust redistribution in blood flow after vascular occlusion.
C. B. Schaffer, B. Friedman, N. Nishimura, L. F. Schroeder, P.
S. Tsai, F. F. Ebner, P. D. Lyden and D. Kleinfeld. Public Library
of Science: Biology (2006) 4:258-270
PDF (4.6 Mb) ; supplemental information
PDF (6.6 Mb) ; supplemental video
MOV (7.2 Mb) and caption
PDF (0.1 Mb). Synopsis titled "Redundancy in
Cortical Surface Vessels Supports Persistent Blood Flow"
by L. Gross, PLoS: Biology (2006) 2:e46
PDF (0.4 Mb). News release, titled “Laser technique sheds
light on strokes”, featured in Science Daily
PDF (0.3 Mb), Medical News Today, YubaNet.com, What's New in
Health, Innovations Report (Germany), PhysOrg.com, ProHealth.com,
SLF Org News Center, WhatsNextNetwork.com, WebIndia, NewKerala.com,
Science News, ProHealth.com, GlobalRPh.com, AlzheimerSupport.com,
MedGadget.com, and OE Magazine (SPIE). Additionally featured in
“Brain's blood re-routes round clots“ by J. Marshall
in the New Scientist (2006) no. 2534
PDF (0.1 Mb), a news brief by P. Daukantas in Optics &
Photonics News (February 2006), and a news brief by D. Burgess
in Photonics Spectra (February 2006).
Spectroscopy of third harmonic generation: Evidence for
resonances in model compounds and ligated hemoglobin.
G. O. Clay, A. C. Millard, C. B. Schaffer, J. Aus-der-Au, P. S.
Tsai, J. A. Squier and D. Kleinfeld. Journal of the Optical Society
of America B (2006) 23:932-950. PDF
(1.8 Mb)
GABA-B mediated feedback: A mechanism to code stimulus
frequency by latency in the thalamic network. D. Golomb,
E. Ahissar and D. Kleinfeld, Journal of Neurophysiology (2006)
95:1735-1750. PDF
(0.9 Mb)
(2001 - 2005)
From art to engineering? The rise of in vivo mammalian
electrophysiology through genetically targeted labeling and nonlinear
imaging. D. Kleinfeld and O. Griesbeck. PLoS Biology
(2005) 3:1685-1689. PDF
(0.6 Mb)
Related material quoted in "Peering into the brain: New frontiers
in neural imaging" by B. Patoine, Dana Foundation's Brain
Work Neuroscience Newsletter (2005) 15:1-3. PDF
(0.3 Mb)
A femtosecond laser-drilled capillary integrated into
a microfluidic device. T. N. Kim, K. Campbell, A. Groisman,
D. Kleinfeld and C. B. Schaffer. Applied Physics Letters (2005)
86:201106. PDF
(0.3 Mb)
Featured in "Femtosecond laser brings 3-D to microfluidics''
by R. Gaughan, Photonics Spectra (2005) August issue. PDF
(0.9 Mb)
Activation of nucleus basalis facilitates cortical control
of a brainstem motor program. R. W. Berg, B. Friedman,
L. F. Schroeder and D. Kleinfeld. Journal of Neurophysiology (2005)
94:699-711. PDF
(3.9 Mb)
Positive feedback in a brainstem tactile sensorimotor
loop. Q.-T. Nguyen and D. Kleinfeld. Neuron (2005) 45:447-457. PDF
(0.7 Mb)
Featured in "Preview: Navigating a sensorimotor
loop" by E. E. Fanselow and B. W. Connors, Neuron (2005)
45:329-330. PDF (0.1 Mb)
News release “Rat Whiskers Give Clues to Blinking Disorder”
featured at Healthday.com (HealthDayNews) PDF
(0.1 Mb)
and picked up by CommunityMemorial.com (Menomonee Falls, WI),
Forbes.com, GoTriad.com, HealthCentral.com, HealthFinder.gov (US
Dept. HHS), HealthScout.com, Health.Yahoo.com, HON.ch (Health
on the Net), hvlib.Integris-Health.com (Oklahoma City, OK), LifeClinic.com,
NewsTrove.com, NorthShoreLIJ.com (North Shore - Long Island Jewish
Health System, NY), Reflector.com (The Daily Reflector), and WaukeshaMemorial.org.
“Eye Disorder Clue from Rat Whiskers “ featured at
Health24.com (South Africa). “Rat Whisking May Provide Insight
Into Debilitating Eye Disorder” featured at Innovations-Reports.com
(Germany), NewsWise.com, ScienceDaily.com, UrekAlert.org (AAAS),
and MediLexicon.com. PDF
(0.1 Mb).
"A Harry Clue About Blepharospam" featured in EyeOrbit
(American Academy of Opthamology) PDF
(0.1 Mb)
Goal-directed whisking behavior increases phase-locking
between vibrissa movement and electrical activity in primary sensory
cortex in rat. K. Ganguly and D. Kleinfeld. Proceedings
of the National Academy of Sciences USA (2004) 101:12348-12353.
PDF (0.6 Mb)
Supplemental Material. PDF
(0.6 Mb)
Beyond observation: Microscopy with ultrashort laser
pulses to probe and manipulate cortical vasculature.
G. O. Clay, N. Nishimura, C. B. Schaffer, P. S. Tsai and D. Kleinfeld.
SPIE International Technical Group Newsletter, Optics in Information
Systems (2004)15:4-5. PDF
(1.2 Mb)
Current flow in vibrissa motor cortex can phase-lock
with exploratory whisking in rat. K. F. Ahrens and D.
Kleinfeld. Journal of Neurophysiology (2004) 92:1700-1707. PDF
(1.2 Mb)
Featured in "What Makes Whiskers Shake? Focus
on: Current Flow in Vibrissa Motor Cortex Can Phase-Lock With
Exploratory Rhythmic Whisking in Rat'' by M. Brecht, Journal of
Neurophysiology (2004) 92:1265-1266. PDF
(0.1 Mb)
Ultrashort pulsed laser light: A cool tool for ultraprecise
cutting of tissue and cells. P. S. Tsai, B. Friedman,
J. A. Squier and D. Kleinfeld. Optics and Photonics News (2004)
15:24-29. PDF
(1.2 Mb)
Developmental regulation of active and passive membrane
properties in rat vibrissa motoneurons. Q.-T. Nguyen,
R. Wessel and D. Kleinfeld. Journal of Physiology (2004) 556:203-219.
PDF
(0.9 Mb)
Frisking the whiskers: Coding of patterned sensory input
in the rat vibrissa system. S. B. Mehta and D. Kleinfeld.
Neuron (2003) 41:181-184. PDF
(1.0 Mb)
Reversing cerebellar long-term depression. V.
Lev-Ram, S. B. Mehta, D. Kleinfeld and R. Y. Tsien. Proceedings
of the National Academy of Sciences USA (2003) 100:15989-15993.
PDF (0.5 Mb)
Featured in "Synaptic plasticity: Ups and downs of cerebellar
plasticity" by S. Farley, Nature Reviews of Neuroscience 5:85
(2004).
Imaging reveals synaptic targets of a swim-terminating
neuron in the leech CNS. A. Taylor, G. Cottrell, D. Kleinfeld
and W. Kristan, Jr. Journal of Neuroscience (2003) 23:11402-11410.
PDF (0.5 Mb)
Vibrissa movement elicited by rhythmic electrical microstimuation
to motor cortex in the aroused rat mimics exploratory whisking.
R. W. Berg and D. Kleinfeld. Journal of Neurophysiology (2003)
90:2950-2963. PDF
(1.3 Mb)
All-optical histology using ultrashort laser pulses.
P. S. Tsai, B. Friedman, A. I. Ifarraguerri, B. D. Thompson, V.
Lev-Ram, C. B. Schaffer, Q. Xiong, R. Y. Tsien, J. A. Squier and
D. Kleinfeld. Neuron (2003) 39:27-41. PDF
(0.9 Mb)
Featured in "Femtosecond laser for cutting and imaging brain tissue"
by P. Schewe, J. Riordon and B. Stein AIP Bulletin (Physics news
update, 5 June 2003); "Lasers take 3D brain scans: Infrared light
probes tissue a slice at a time" by G. Brumfiel, Nature (Science
update, 10 June 2003); "Ultrafast lasers capture brain images",
Photonics.com (Feature article, 2 July 2003); "Brain breakthrough",
Fox 6 News (2 July 2003) MOV
(7.2 Mb);
"Ultrafast lasers slice up high-quality brain tissue images",
Space Daily (7 July 2003); "All-optical histology with femtosecond
lasers" by S. Benka, Physics Today (Physics update, August 2003);
"Ultrafast lasers enable all-optical histology" by D. Burgess,
Photonics Spectra (August 2003); "All-optical histology using
ultra-short laser pulses" by D. Boas, Photonics International
(September 2003); "Mind-reader" by R. Smallridge, Nature Reviews
- Molecular cell Biology (September 2003) PDF
(0.2 Mb);
"Newest imaging methods begin 'golden age' of seeing inside body",
by R. Ehrenberg, The Dallas Morning News (October 2003) PDF
(0.2 Mb);
"Slice of Life: Researchers develop new techniques to unveil the
architecture of tissues", by K. Heyman , The Scientist (November
2003) PDF
(0.3 Mb).
Unilateral vibrissa contact: Changes in amplitude but
not the timing of vibrissa movement. R. H. S. Sachdev,
R. W. Berg, G. Chompney, D. Kleinfeld and F. F. Ebner. Somatosensory
and Motor Research (2003) 20:162-169. PDF
(0.2 Mb)
Rhythmic whisking in rat: Retraction as well as protraction
is under active muscular control. R. W. Berg and D. Kleinfeld.
Journal of Neurophysiology (2003) 89:104-117. PDF
(0.8 Mb)
Closed loop neuronal computations: Focus on vibrissa
somatosensation in rat. E. Ahissar and D. Kleinfeld.
Cerebral Cortex (2003) 13:53-62. PDF
(0.5 Mb)
Spectral mixing of rhythmic neuronal signals in sensory
cortex. K. F. Ahrens, H. Levine, H. Suhl and D. Kleinfeld.
Proceedings of the National Academy of Sciences USA (2002) 99:15176-15181.
PDF
(0.4 Mb)
Adaptive filtering of vibrissa input in motor cortex
of rat. D. Kleinfeld, R. N. S. Sachdev, L. M. Merchant,
M. R. Jarvis and F. F. Ebner. Neuron (2002) 34:1021-1034. PDF
(0.7 Mb)
Featured in "How does the human brain regulate sensory information",
Medical Device and Diagnostic Industry (R&D Digest, July 2002).
Coherent electrical activity between vibrissa sensory
areas of cerebellum and neocortex is enhanced during free whisking.
S. M. O'Connor, R. W. Berg and D. Kleinfeld. Journal of Neurophysiology
(2002) 87:2137-2148. PDF
(1.3 Mb)
Cortical imaging through the intact mouse skull using
two-photon excitation laser scanning microscopy. E. J.
Yoder and D. Kleinfeld. Microscopy Research and Technique (2002)
56:304-305. PDF
(0.3 Mb)
Traveling electrical waves in cortex: Insights from phase
dynamics and speculation on a computational role. G.
B. Ermentrout and David Kleinfeld. Neuron (2001) 29:33-44. PDF
(1.0 Mb)
Chattering and differential signal processing in identified
motion-sensitive neurons of parallel visual pathways in the chick
tectum. H. Luksch, H. J. Karten, D. Kleinfeld, and R.
Wessel. Journal of Neuroscience (2001) 15:6440-6446. PDF
(0.3 Mb)
